GMO Safety Assessment-Feasibility of Bioassay to Detect Allelopathy Using Handy Sandwich Method in Transgenic Plants
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منابع مشابه
Assessment of salt tolerance in transgenic tobacco (Nicotiana tobacum L.) plants expressing the AUX gene
Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and plants regenerated fro...
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Cotton cultivar Coker has been already transformed with recombinant pBI121-chi via Agrobacterium tumefaciens. The T-DNA region of pBI121-chi carries the chitinase (chi ) gene from bean and is under the control of the CaMV35S promoter. T1 and T2 progenies of transgenic cotton containing the chi gene were used in this study. Polymerase chain reaction (PCR), Southern and Western blotting data con...
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Risk assessment of a transgene is one of the key steps in genetic transformation. Hence, in order to use cryIAc gene for production of transgenic plants, a library and in-silico research was performed to confirm safety of the gene for human comsumption, animal feed and environment. In the first step, the molecular mechanism of action of the CryIAc protein and its specific receptors in the midgu...
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Tomato (Solanum lycopersicum L.) is an important vegetable in the world, it exposes to a wide range of pathogens and plant pests attack. Fruit worm is one of the most important the plant pest which mainly damage the fruit and causes the yield reduction. In this study, cry1a105 gene was cloned to pBI121 plasmid (with 35S promoter and Nos terminator), and was transferred to tomato by Agrobacteriu...
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